Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Bacterial microcolony and its primary applications].

X Pei, M Ye, Q Yin

    Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
    |September 1, 1992
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mitochondrial DNA deletions parallel age-linked decline in rat sensory nerve function.

    Neurobiology of aging·2001
    Same author

    Ruthenium-catalyzed oxidative cleavage of olefins to aldehydes.

    The Journal of organic chemistry·2001
    Same author

    Pharmacokinetics of testosterone after percutaneous gel or buccal administration.

    Fertility and sterility·2001
    Same author

    The leukocyte common antigen-related protein tyrosine phosphatase receptor regulates regenerative neurite outgrowth in vivo.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
    Same author

    Calpain activity in the rat brain after transient forebrain ischemia.

    Experimental neurology·2001
    Same author

    Sharpening low-energy, standard-model tests via correlation coefficients in neutron beta decay.

    Physical review letters·2001
    Same journal

    [Determination of warfarin in plasma by HPLC and an investigation of monitoring patients after cardiac valve replacement].

    Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao·2003
    Same journal

    [Improved APAAP immunocytochemical staining-trace cytotoxicity test plate method for detection of cell surface antigens].

    Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao·2003
    Same journal

    [Clinical application and evaluation of polymerase chain reaction-microwell plate hybridization assay in the detection of Mycobacterium tuberculosis].

    Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao·2003
    Same journal

    [Study on the chemosensitivity test of human gastric cancer using the MTT assay].

    Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao·2003
    Same journal

    [Investigation on the value of applying interventional radiologic treatment in the management of advanced gynecological malignancies].

    Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao·2003
    Same journal

    [Study on bolus cyclosphamide treatment for 64 cases of lupus nephritis].

    Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao·2003
    See all related articles

    A novel microcolony technique using cellulose-acetate membranes offers rapid bacterial identification and enumeration. This method accurately differentiates Staphylococcus strains and quantifies bacteria in urine within six hours.

    Area of Science:

    • Microbiology
    • Cellulose Acetate Membrane Technology

    Context:

    • Traditional bacterial identification and enumeration methods can be time-consuming.
    • Accurate and rapid bacterial detection is crucial in clinical diagnostics and research.

    Purpose:

    • To develop and validate a rapid microcolony technique for bacterial observation and quantification.
    • To assess the efficacy of the technique in differentiating bacterial strains and determining bacterial load in biological samples.

    Summary:

    • A new microcolony technique utilizes cellulose-acetate membranes for bacterial cultivation, fixation, and microscopic observation.
    • The method successfully observed microcolonies from 139 bacterial strains, differentiating 72 Staphylococcus strains with 90.28% correlation to routine methods.
    • This technique was also applied for rapid (within 6 hours) bacterial enumeration in urine samples, demonstrating significant advantages over the plate count method.

    Related Experiment Videos

    Impact:

    • Provides a faster alternative for bacterial identification and strain differentiation.
    • Enables quicker diagnosis and monitoring of bacterial infections, particularly in urine samples.
    • Offers a valuable tool for microbiological research requiring rapid bacterial assessment.