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Related Experiment Videos

[Control of gene expression by antisense nucleic acids].

B Lebleu1, J P Clarenc, G Degols

  • 1Université Montpellier II.

Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales
|January 1, 1992
PubMed
Summary

Antisense technology offers precise control over gene expression using antisense RNA or oligonucleotides. Strategies like poly (L-lysine) conjugation and targeted liposomes enhance oligonucleotide delivery and bioavailability for therapeutic applications.

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Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • Oligonucleotide Chemistry

Context:

  • Antisense RNA and oligonucleotides are rapidly developing tools for specific gene control.
  • Advances in oligonucleotide chemistry have yielded analogs with enhanced pharmacological properties.
  • Existing gene expression control methods include antisense, anti-gene, and sense approaches.

Purpose:

  • To discuss the advantages and drawbacks of antisense RNA and oligonucleotide applications.
  • To explore strategies for improving the delivery and bioavailability of synthetic oligonucleotides.
  • To present the authors' research on enhancing oligonucleotide delivery via poly (L-lysine) conjugation and antibody-targeted liposomes.

Summary:

  • The review covers the use of antisense RNA and oligonucleotides for controlling viral and cellular gene expression.

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  • It details progress in oligonucleotide chemistry, leading to improved synthetic analogs.
  • The paper discusses various strategies, including antisense, anti-gene, and sense approaches, and addresses the challenge of oligonucleotide delivery to cellular targets, presenting the authors' work on improving bioavailability.

    • Impact:

    • Provides a comprehensive overview of antisense technology for gene expression modulation.
    • Highlights key challenges in oligonucleotide delivery and bioavailability.
    • Showcases innovative strategies for enhancing the therapeutic potential of antisense oligonucleotides.