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45Ca-efflux in embryonic chick heart and its modification by caffeine and ryanodine

P Prakash1, P Meera, O Tripathi

  • 1Physiology Division, Central Drug Research Institute, Lucknow, India.

Journal of Developmental Physiology
|December 1, 1992
PubMed

Insights

This study reveals how cellular calcium handling in chick hearts changes during development. Key calcium compartments shift, with the sarcoplasmic reticulum becoming more prominent in later embryonic stages.

Area of Science:

  • Cardiovascular Physiology
  • Developmental Biology
  • Cellular Biology

Background:

  • Cellular calcium (Ca2+) handling is crucial for cardiac function.
  • Understanding ontogenic changes in Ca2+ kinetics is vital for comprehending heart development.

Purpose of the Study:

  • To investigate the ontogenic changes in the kinetics of exchangeable cellular calcium in embryonic and post-hatch chick ventricular tissue.
  • To identify distinct cellular Ca2+ compartments and their developmental regulation.

Main Methods:

  • Utilized 45Ca-efflux technique to monitor calcium kinetics in embryonic (ECV) and post-hatch (PHCV) chick ventricular tissue.
  • Analyzed efflux curves to identify three kinetically distinct Ca2+ compartments (C1, C2, C3).
  • Assessed the effects of caffeine and ryanodine on Ca2+ efflux rates at different developmental stages.

Main Results:

  • Identified three kinetically distinct Ca2+ compartments (C1, C2, C3) with varying sizes and rate constants.
  • Observed a significant decrease in the size of compartment C1 and an increase in C3 size with development.
  • Demonstrated increased fractional escape rates with caffeine and ryanodine, particularly in later developmental stages, indicating sarcoplasmic reticulum (SR) prominence.

Conclusions:

  • The study indicates significant developmental shifts in cellular Ca2+ compartments within chick ventricular tissue.
  • The increasing size of compartment C3 suggests differentiation and growing importance of the sarcoplasmic reticulum (SR) during late embryogenesis.
  • Pharmacological interventions confirm the age-dependent development and functional significance of the SR in calcium handling.

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