Experimental studies of pancreatic transplantation after freezing and thawing

The successful rat pancreatic transplantation, after 30 minutes cryopreservation at -80 degrees C, is presented with the normal graft function till the 6th postoperative day maximum. The freezing device used by the authors is shortly described. The choice of optimal freezing and thawing parameters is discussed. Organ freezing is a hopeful method which could serve to preserve the solid organs for a long period of time. Our former experimental research showed that slow freezing (1-2 degrees C/min) and fast thawing (> 100 degrees C/min) had produced less damage to the pancreas of the rat [1]. Recently we performed rat pancreatic transplantation after freezing and thawing the donor pancreas with the parameters mentioned above.