Related Experiment Video

Updated: Aug 17, 2026

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Published on: July 13, 2013

Resolution and reconstitution of ion-transport systems

E Racker, A F Knowles, E Eytan

    Annals of the New York Academy of Sciences
    |December 30, 1975
    PubMed

    More Related Videos

    Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein09:59

    Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

    Published on: March 9, 2015

    Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters11:51

    Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

    Published on: February 3, 2018

    Related Concept Videos

    Secondary Active Transport01:32

    Secondary Active Transport

    One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
    Ion Exchange01:17

    Ion Exchange

    Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
    Ion-Exchange Chromatography01:09

    Ion-Exchange Chromatography

    Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
    Voltage-gated Ion Channels01:26

    Voltage-gated Ion Channels

    Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
    Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
    Transport Number01:31

    Transport Number

    The transport number is the fraction of the total current carried by an ion in an electrolyte solution. It is defined as the ratio of the current carried by a specific ion to the total current flowing through the solution. The transport number, t, is central to understanding ionic mobility, which describes how fast an ion moves under the influence of an electric field. This link connects the physical behavior of ions in solution to the chemical processes that occur during electrochemical...
    Pore Transport and Ion-Pair Transport01:17

    Pore Transport and Ion-Pair Transport

    Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
    Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited  but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct microscopic...