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Proteolytic activity during storage of platelets in plasma
J Wallvik1, A M Suontaka, M Blombäck
1Department of Clinical Chemistry and Blood Coagulation, Karolinska Hospital, Stockholm, Sweden.
Transfusion Medicine (Oxford, England)
|June 1, 1992
Summary
White blood cells in platelet concentrates (PC) increase proteolytic activity during storage. Higher white cell counts correlate with increased elastase and spontaneous proteolytic activity, impacting PC quality.
Area of Science:
- Transfusion Medicine
- Hematology
- Biochemistry
Background:
- Platelet concentrates (PC) are stored in plasma at 22°C.
- White blood cell concentration in PC can vary.
- Assessing proteolytic activity is crucial for PC quality.
Purpose of the Study:
- To investigate proteolytic activity in stored platelet concentrates (PC).
- To determine the correlation between white cell concentration and proteolytic markers.
- To assess the metabolic status of PC during storage.
Main Methods:
- Prepared PC with high (A) and low (B) white cell concentrations.
- Stored cell-free plasma (PPP) as a control.
- Assayed fibrinopeptide A (FPA), elastase, spontaneous proteolytic activity (SPA), thrombin-antithrombin complexes (TAT), and D-dimers.
- Monitored glucose, pH, lactate dehydrogenase (LDH), and ATP levels.
Main Results:
- Decreased pH correlated with higher leucocyte count and LDH.
- Elastase and SPA levels were low in PPP but increased in PC.
- PC with higher white cell counts (PC A) showed significantly higher elastase and SPA.
- Leucocyte count correlated with elastase and SPA.
- Minor FPA increase observed; no TAT or D-dimers detected.
Conclusions:
- White blood cells contribute to increased proteolytic activity in stored PC.
- Higher white cell concentrations exacerbate proteolytic enzyme activity.
- Proteolytic activity, particularly elastase and SPA, is influenced by leucocyte content in PC.