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Related Experiment Videos

Platelet counting using plasma platelet concentrate samples.

R A Lord1, G A Smith, M J Nightingale

  • 1Wessex Regional Transfusion Centre, Southampton, U.K.

Transfusion Medicine (Oxford, England)
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

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Accurate platelet counting is crucial for transfusion safety. Different automated counters, like the Coulter S Plus and Sysmex E-2500, show varying accuracy and linearity with platelet-rich plasma, impacting platelet yield determination.

Area of Science:

  • Transfusion Medicine
  • Hematology
  • Laboratory Science

Background:

  • Accurate platelet counting is essential for ensuring the efficacy and safety of platelet transfusions.
  • Automated cell counters are widely used for platelet enumeration, but their performance with high-concentration samples requires careful validation.

Purpose of the Study:

  • To evaluate the accuracy and linearity of different automated platelet counting methods using plasma from platelet concentrates.
  • To compare the performance of Coulter S Plus and Sysmex E-2500 counters against a manual reference method.

Main Methods:

  • Platelet counts were performed using a manual phase-contrast microscopy method.
  • Automated counting was conducted using Coulter S Plus and Sysmex E-2500 instruments.
  • Analysis included assessment of reproducibility, accuracy, and linearity of counts in neat and pre-diluted platelet concentrate samples.

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Main Results:

  • All methods demonstrated good reproducibility (CVs: 4.9%, 2.2%, 1.4% for manual, Coulter, Sysmex, respectively).
  • The Coulter S Plus counter yielded inaccurate and non-linear results for neat platelet concentrate samples above 900 x 10(9)/l.
  • The Sysmex E-2500 counter provided accurate and linear counts without pre-dilution, comparable to the manual method.

Conclusions:

  • The choice of automated platelet counting method significantly influences the accuracy of platelet yield assessment in transfusion products.
  • Pre-dilution is necessary for accurate Coulter S Plus counting of platelet concentrates, while the Sysmex E-2500 offers reliable results without it.
  • Method-dependent variations in platelet counting can affect the determination of true platelet yields.