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A new continuous optical assay for maltase and sucrase
M Giorgi1, P Vanni, G Pinzauti
1Dipartimento di Scienze Biochimiche, Firenze, Italia.
Enzyme
|January 1, 1992
Summary
A novel enzymatic assay for maltase and sucrase activity was developed. This method utilizes mutarotase, hexokinase, and glucose 6-phosphate dehydrogenase for accurate enzyme quantification.
Area of Science:
- Biochemistry
- Enzymology
Background:
- Maltase and sucrase are crucial enzymes involved in carbohydrate metabolism.
- Accurate quantification of these enzymes is essential for various biochemical and clinical applications.
Purpose of the Study:
- To report a new, efficient method for the quantitative assay of maltase and sucrase activity.
- To validate the linearity and reliability of the developed assay.
Main Methods:
- The assay employs a coupled enzymatic system involving mutarotase, hexokinase, and glucose 6-phosphate dehydrogenase.
- Spectrophotometric monitoring of reaction progress was utilized.
Main Results:
- The developed method provides a reliable assay for both maltase and sucrase.
- The enzymatic reaction demonstrated linearity up to a change in absorbance per minute (ΔE/min) of 0.13, indicating a robust assay range.
Conclusions:
- A novel and effective enzymatic assay for maltase and sucrase has been established.
- The assay's linearity ensures its suitability for accurate enzyme quantification in various biological samples.