Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insertion and excision of Bacteroides conjugative chromosomal elements.

L A Bedzyk1, N B Shoemaker, K E Young

  • 1Department of Microbiology, University of Illinois, Urbana 61801.

Journal of Bacteriology
|January 1, 1992
PubMed
Summary

Large Bacteroides chromosomal elements, often carrying tetracycline resistance (Tcr) genes, can transfer between bacteria. These elements exhibit non-random insertion and can move unlinked DNA segments, with specific sequences potentially mediating their integration and excision.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact Melt Facies in the Moon's Crisium Basin: Identifying, Characterizing, and Future Radiogenic Dating.

Journal of geophysical research. Planets·2020
Same author

The perverse impact of external reference pricing (ERP): a comparison of orphan drugs affordability in 12 European countries. A call for policy change.

Journal of market access & health policy·2017
Same author

Boosting Biosimilars Uptake in European Countries.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

An Overview of the Biosimilar Market in the Us.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

HTA Approach in Italy. Structure, Methods, And Process Of Veneto Region's Evaluation Of Pharmaceutical Effectiveness Unit (Unità Di Valutazione Dell'efficacia Del Farmaco, Uvef).

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

Gene expression in Escherichia coli biofilms.

Applied microbiology and biotechnology·2004

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Bacteroides species possess large, self-transferable chromosomal elements.
  • Many of these elements confer tetracycline resistance (Tcr).
  • These mobile elements can influence bacterial genome evolution.

Purpose of the Study:

  • To characterize the size and insertion behavior of Tcr elements in Bacteroides.
  • To investigate the mechanism of insertion and excision of these elements.
  • To identify DNA sequences involved in the mobility of these elements.

Main Methods:

  • Transverse alternating field electrophoresis (TAFE) for size determination.
  • DNA sequencing of junction regions between elements and target sites.
  • Analysis of element insertion and excision events.

Related Experiment Videos

Main Results:

  • Tcr elements are typically 70-80 kbp, with one exception at 150-200 kbp.
  • Insertion of Tcr elements and associated nonreplicating Bacteroides units (NBUs) is non-random.
  • Sequence analysis revealed no target site duplication upon insertion and identified a potential recombinational hot spot sequence.
  • Excision from plasmids was observed, often with minor target site alterations.

Conclusions:

  • Bacteroides Tcr elements are large mobile genetic elements with complex integration and transfer mechanisms.
  • Specific DNA sequences, including a potential hot spot, likely mediate element mobility.
  • These elements can facilitate the transfer of chromosomal DNA, impacting bacterial genetics.