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Partite expression of the bovine papillomavirus E1 open reading frame in Escherichia coli
1Department of Medical Microbiology and Immunology, College of Medicine, Texas A & M University, College Station 77843.
Biochimica Et Biophysica Acta
|January 6, 1992
Abstract:
Six recombinants were constructed which expressed portions of the bovine papillomavirus E1 open reading frame as OmpF/E1/beta-galactosidase tribrid fusion proteins in Escherichia coli. Rabbit sera containing E1-specific antibodies were generated against five of these six fusion proteins (which together constitute 74% of the full-length E1 open reading frame). The individual fusion proteins and their cognate antisera will be useful reagents for defining the structure and function of the BPV E1 protein(s).