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Expression of feline immunodeficiency virus gag gene in Escherichia coli
T Furuya1, A Hasegawa, M Saitoh
1Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Archives of Virology
|January 1, 1992
Abstract:
The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.