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Rotational motion of monomeric and dimeric immunoglobulin E-receptor complexes
J N Myers1, D Holowka, B Baird
1Department of Chemistry, Baker Laboratory, Cornell University, Ithaca, New York 14853-1301.
Biochemistry
|January 21, 1992
Summary
This study investigated the rotational dynamics of immunoglobulin E (IgE) receptors on rat basophilic leukemia cells. Dimeric IgE-receptor complexes interact with membrane components on intact cells, affecting their mobility.
Area of Science:
- Biophysics
- Cell Biology
- Immunology
Background:
- Immunoglobulin E (IgE) receptors play a crucial role in allergic responses.
- Understanding receptor dynamics is key to deciphering cell signaling pathways.
Purpose of the Study:
- To investigate the rotational dynamics of monomeric and dimeric Fc epsilon RI receptors for IgE.
- To compare receptor dynamics on living RBL cells versus membrane vesicles.
Main Methods:
- Utilized time-resolved phosphorescence anisotropy with erythrosin 5'-thiosemicarbazide labeled IgE.
- Studied receptors on both intact rat basophilic leukemia (RBL) cells and derived membrane vesicles.
Main Results:
- Monomeric IgE-receptor complexes showed rotational correlation times consistent with free rotation, with a potential immobile fraction on cells.
- Dimeric IgE-receptor complexes were largely immobile on cells but mobile on vesicles, with increased rotational correlation time.
- A faster rotational motion component was observed on vesicles, suggesting segmental flexibility within the receptor.
Conclusions:
- Dimeric IgE-receptor complexes interact with other membrane components on intact cells, unlike on vesicles.
- These interactions likely influence receptor function and signaling in RBL cells.
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