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Related Experiment Videos

Distinct subsets of retroviruses encode dUTPase.

J H Elder1, D L Lerner, C S Hasselkus-Light

  • 1Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037.

Journal of Virology
|March 1, 1992
PubMed
Summary

Nonprimate lentiviruses possess a unique proteaselike element gene absent in primate lentiviruses. This element confers dUTPase activity, crucial for viral replication, as demonstrated in feline immunodeficiency virus.

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Area of Science:

  • Virology
  • Molecular Biology
  • Enzymology

Background:

  • Nonprimate lentiviruses (feline immunodeficiency virus, equine infectious anemia virus, visna virus, caprine encephalitis virus) possess a unique gene segment within their polymerase gene, absent in primate lentiviruses.
  • This gene segment, termed proteaselike element, shows sequence homology to aspartate proteinase and dUTPase enzyme families.
  • Related sequences are also found in other retroviruses, particularly type D retroviruses.

Purpose of the Study:

  • To investigate the enzymatic activity associated with the proteaselike element in nonprimate lentiviruses and type D retroviruses.
  • To demonstrate that the proteaselike element in feline immunodeficiency virus encodes dUTPase activity.

Main Methods:

  • Bioinformatic analysis using computer searches to identify sequence relatedness.

Related Experiment Videos

  • Enzymatic assays to detect dUTPase activity in nonprimate lentiviruses and type D retroviruses.
  • Genetic analysis in feline immunodeficiency virus to confirm the proteaselike element's role in encoding dUTPase activity.

    • Main Results:

    • Both nonprimate lentiviruses and type D retroviruses exhibit dUTPase activity.
    • The proteaselike element in feline immunodeficiency virus is formally demonstrated to encode this dUTPase activity.

    Conclusions:

    • The proteaselike element represents a functional gene encoding dUTPase activity in nonprimate lentiviruses and type D retroviruses.
    • This finding highlights a significant molecular difference between primate and nonprimate lentiviruses, potentially impacting viral replication and pathogenesis.