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Expression of herpes simplex virus type 1 glycoprotein B in insect cells. Initial analysis of its biochemical and
H Ghiasi1, R Kaiwar, A B Nesburn
1Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
Abstract:
A recombinant baculovirus (vAc-gB1) was constructed which expresses the glycoprotein B (gB) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with these recombinant viruses, a protein that was close in size to authentic HSV-1 gB was detected by gB polyclonal antibody. The recombinant gB was found on the membrane of Sf9 cells and was susceptible to tunicamycin, glycosidase F (PNGase F) and partially susceptible to Endo-H. Antibodies raised in mice to this recombinant recognized viral gB and neutralized the infectivity of HSV-1 in vitro. Mice inoculated with the recombinant gB were protected from lethal challenge with HSV-1.