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Related Experiment Videos

Plasminogen binding sites in normal human skin.

S M Burge1, J M Marshall, S A Cederholm-Williams

  • 1Department of Dermatology, Slade Hospital, Oxford, U.K.

The British Journal of Dermatology
|January 1, 1992
PubMed
Summary
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Epidermal cells bind plasminogen (Pg) through specific sites. This binding involves the kringle 5 aminohexyl (AH) site and lysine binding sites on kringles 1-3, facilitating cell functions.

Area of Science:

  • Dermatology
  • Biochemistry
  • Cell Biology

Background:

  • Plasminogen (Pg) is present in the epidermis.
  • Keratinocytes produce plasminogen activators.
  • Plasmin generation may influence keratinocyte behavior.

Purpose of the Study:

  • Investigate plasminogen binding sites in normal human epidermis.
  • Characterize the interactions involved in plasminogen uptake by epidermal cells.

Main Methods:

  • Displacement assays using 6-aminohexanoic acid and benzamidine.
  • Incubation of epidermal sections with various forms of plasminogen and its fragments.
  • Analysis of plasminogen binding characteristics.

Main Results:

Related Experiment Videos

  • Endogenous epidermal plasminogen is displaced by 6-aminohexanoic acid and benzamidine.
  • Initial plasminogen binding occurs via the kringle 5 aminohexyl (AH) site.
  • Secondary interaction involves lysine binding sites on kringles 1-3.
  • Cell binding is independent of plasmin active site reactions.

    • Conclusions:

    • Plasminogen binding to human epidermis is a multi-step process.
    • Specific molecular domains of plasminogen mediate epidermal cell interactions.
    • Understanding these interactions is crucial for epidermal biology.