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Expression of the heparin-binding growth factor receptor genes in human megakaryocytic leukemia cells

O Katoh1, Y Hattori, T Sato

  • 1Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

Biochemical and Biophysical Research Communications
|February 28, 1992
PubMed

Insights

This study identifies K-sam/bek, N-sam/flg, and FGFR3/sam3 receptors in human leukemia cells. These findings suggest heparin-binding growth factors (HBGFs) play a role in megakaryocytopoiesis and platelet function.

Area of Science:

  • Molecular biology
  • Hematology
  • Cell signaling

Background:

  • K-sam/bek, N-sam/flg, and FGFR3/sam3 receptors form a gene family for heparin-binding growth factors (HBGFs)/fibroblast growth factors (FGFs).
  • These receptors are crucial for cellular communication and development.

Purpose of the Study:

  • To investigate the presence and role of HBGF receptors in human leukemia cells with megakaryocytic potential.
  • To explore the involvement of HBGFs in megakaryocytopoiesis and platelet function.

Main Methods:

  • Detection of K-sam/bek, N-sam/flg, and FGFR3/sam3 mRNAs in human leukemia cell lines (CMK, K562, HEL).
  • Analysis of N-sam/flg transcript levels in CMK cells after 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulation.
  • cDNA-polymerase chain reaction to identify K-sam/bek mRNA in human platelets.

Main Results:

  • K-sam/bek, N-sam/flg, and FGFR3/sam3 mRNAs were detected in human leukemia cell lines (CMK, K562, HEL) exhibiting megakaryocytic characteristics.
  • N-sam/flg transcript levels increased in CMK cells upon TPA treatment.
  • K-sam/bek mRNA was identified in human platelets.

Conclusions:

  • The HBGF receptor gene family is present in human leukemia cells with megakaryocytic lineage potential.
  • HBGFs likely contribute to megakaryocytopoiesis and the functions of platelets.

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