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Voltage-dependent calcium entry in confluent bovine capillary endothelial cells
J L Bossu1, A Elhamdani, A Feltz
1Laboratoire d'Etude des Régulations Physiologiques, CNRS associé à l'Université Louis Pasteur, Strasbourg, France.
FEBS Letters
|March 16, 1992
Summary
Confluent bovine capillary endothelial cells have two calcium channel types sensitive to BAY K 8644. These channels require depolarization or specific substances to activate, unlike isolated cells.
Area of Science:
- Cellular physiology
- Ion channel biophysics
Background:
- Endothelial cells play crucial roles in vascular function.
- Voltage-dependent calcium channels regulate cellular processes.
Purpose of the Study:
- To characterize voltage-dependent calcium channels in confluent bovine capillary endothelial cells.
- To investigate the activation properties of these channels.
Main Methods:
- Cell-attached patch-clamp recordings were used.
- Barium ions (Ba2+) served as the charge carrier.
- The dihydropyridine calcium agonist BAY K 8644 was applied.
Main Results:
- Two types of calcium channels (4 pS and 23.5 pS) were identified.
- Both channel types were sensitive to the calcium agonist BAY K 8644.
- Confluent cells showed no low-threshold (T-type) activity and required strong depolarization for calcium current activation.
Conclusions:
- Confluent endothelial cells possess specific dihydropyridine-sensitive calcium channels.
- Activation of these channels is shifted towards more depolarized potentials compared to isolated cells.
- Modulation of channel activation is necessary for calcium entry in confluent endothelial cells.