Alternative multimeric structures affect myogenin DNA binding activity.

¹Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235.

|March 15, 1992

Summary

Muscle-specific basic helix-loop-helix (bHLH) proteins form large complexes, not just dimers. These higher-order structures may limit their DNA-binding activity, impacting skeletal muscle development.

Related Concept Videos

You might also read

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026

Same author

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026

Same author

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026

Area of Science:

Molecular Biology
Protein Biochemistry
Developmental Biology

Background:

Basic helix-loop-helix (bHLH) proteins are crucial transcription factors in muscle development.
Understanding the oligomeric state of bHLH proteins is key to their DNA-binding and regulatory functions.

Purpose of the Study:

To determine the native molecular weight and oligomeric states of myogenin, MyoD, and E12.
To investigate how these oligomeric states affect DNA binding.
To elucidate the regulatory mechanisms of skeletal muscle bHLH proteins during myogenesis.

Main Methods:

Sucrose gradient sedimentation
Molecular sieve chromatography
Analysis of purified proteins and nuclear extracts from differentiated myotubes.

Main Results:

Muscle bHLH proteins (myogenin, MyoD) form higher-order complexes beyond dimers.
Homodimers bind DNA (e.g., MEF-1 site), but homotetramers and larger complexes do not.
Myogenin predominantly exists in large, non-DNA-binding complexes in vitro and in vivo.
A model involving chain or ring structures is proposed to explain complex formation.

Conclusions:

Myogenin's association into large complexes influences its DNA-binding activity.
The partitioning of myogenin into different oligomeric states presents novel regulatory mechanisms for myogenesis.
Further research is needed to fully understand the implications of these higher-order complexes in skeletal muscle differentiation.

Related Experiment Videos

Alternative multimeric structures affect myogenin DNA binding activity.

Related Concept Videos

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

First Observation of the B[over ¯]_{s}^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay and Evidence for the B[over ¯]^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay.

Related Experiment Videos

Alternative multimeric structures affect myogenin DNA binding activity.

Related Concept Videos

Related Articles

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

First Observation of the B[over ¯]_{s}^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay and Evidence for the B[over ¯]^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay.

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions: