Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An SV40 transformation revertant due to a host mutation: isolation and complementation analysis.

G H Silverstein1, D C Kohrman, J B Christensen

  • 1Graduate Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor 48109-0620.

Virology
|April 1, 1992
PubMed
Summary

Researchers identified a new cell line, CL1L, resistant to simian virus 40 (SV40) transformation. This resistance is due to a recessive cellular mutation, suggesting distinct genetic pathways regulate SV40 transformation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Ultra-high dose rate dosimetry for pre-clinical experiments with mm-small proton fields" [Phys. Med. 104 (2022) 101-111].

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2025
Same author

Dosimetry of ultra-high dose rate electron beams using thermoluminescence and optically stimulated luminescence detectors.

Physics in medicine and biology·2024
Same author

Investigation of TL and OSL detectors in ultra-high dose rate electron beams.

Physics in medicine and biology·2023
Same author

Characterization of LiF:Mg,Ti thermoluminescence detectors in low-LET proton beams at ultra-high dose rates.

Physics in medicine and biology·2023
Same author

Ultra-high dose rate dosimetry for pre-clinical experiments with mm-small proton fields.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2022
Same author

Mapping initial and general recombination in scanning proton pencil beams.

Physics in medicine and biology·2020

Area of Science:

  • Cell Biology
  • Virology
  • Cancer Research

Background:

  • Simian virus 40 (SV40) is a DNA tumor virus that can induce cellular transformation.
  • Understanding the cellular mechanisms underlying SV40-induced transformation is crucial for cancer research.

Purpose of the Study:

  • To isolate and characterize a novel cell line resistant to SV40 transformation.
  • To investigate the genetic basis of SV40 transformation resistance.

Main Methods:

  • Selection of SV40 transformation revertant cell line (CL1L) under low serum conditions.
  • Fusion experiments between CL1L cells and normal or SV40-transformed cells.
  • Complementation analysis using another SV40-resistant cell line (A27).

Main Results:

Related Experiment Videos

  • CL1L cells express wild-type SV40 large T antigen but are resistant to transformation.
  • Resistance to SV40 transformation in CL1L is recessive, as indicated by transformed somatic cell hybrids.
  • Fusion of CL1L with A27 cells resulted in transformed hybrids, suggesting complementation and distinct resistance mechanisms.

Conclusions:

  • A cellular mutation confers recessive resistance to SV40 transformation in CL1L cells.
  • The findings indicate the existence of multiple, distinct genetic complementation groups involved in SV40 transformation resistance.