[The long-term effects of cyclosporine on cultured rat mesangial cell]

In recent years, cyclosporine A(CSA) had been extensively used for anti-rejection in organ transplantation. In a high dosage, acute renal failure may occur. In this study, we cultured Sprague-Dawley rat mesangial cells in sandimmune (CSA 1 microgram/ml) and cremophor (13 micrograms/ml) for 2-4 weeks respectively, as the model for a CSA treated patient. A series of experiments were done that included PGE2/cAMP syntheses, mesangial cell proliferation and contraction. The mesangial cells were stimulated with: 1 microM ANGII, 1 microM AVP, 1 microM A23187, 1U/ml IL-1, 25ng/ml PDGF, 1mM IBMX, 10 microM forskolin and 5% fetal bovine serum (FBS). Experimental results were compared between the two groups with the Wilcoxon paired test. The only significant difference was in the cell proliferation study. In conclusion, cell proliferation was inhibited by CSA (p less than 0.05), but the influences of PGE2/cAMP synthesis and cell contraction were minimal on mesangial cells that had been cultured by a CSA contained medium for a relative long period.