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Updated: Aug 18, 2026

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
Published on: February 10, 2015
[Changes of the insulin and glucagon receptors in bile-duct ligated rats]
1First Department of Surgery, Okayama University Medical School, Japan.
Abstract:
The specific bindings of insulin and glucagon to 40000g pellets including hepatic plasma membrane were measured at 1, 2 or 4 weeks after bile-duct ligation or sham operation in rats. At 1 week after operation, insulin bindings were similar in both bile-duct ligated and control rats, however, glucagon binding in bile-duct ligated rats (30.1 +/- 5.99%) was significantly (p less than 0.01) lower than that in control rats (42.8 +/- 8.13%). At 2 and 4 weeks after operation, insulin bindings in bile-duct ligated rats (2 weeks: 35.2 +/- 3.32%, 4 weeks: 32.3 +/- 4.62%) were significantly (p less than 0.05) lower than those in control rats (2 weeks: 39.7 +/- 5.12%, 4 weeks: 38.4 +/- 3.85%). Therefore, glucagon bindings in bile-duct ligated rats (2 weeks: 26.3 +/- 4.32%, 4 weeks: 26.9 +/- 4.06%) were significantly (p less than 0.01) lower than those in control rats (2 weeks: 42.0 +/- 3.95%, 4 weeks: 45.3 +/- 4.29%). Decrease of insulin bindings, however, was less than that of glucagon bindings. Changes of the insulin and glucagon receptors on the hepatic plasma membrane suggested a reason for a disturbance of carbohydrate metabolism in the liver cell with obstructive jaundice.
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