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Related Experiment Videos

Amyloid enhancing factor activity is associated with ubiquitin.

K Alizadeh-Khiavi1, J Normand, S Chronopoulos

  • 1Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

Virchows Archiv. A, Pathological Anatomy and Histopathology
|January 1, 1992
PubMed
Summary

Amyloid enhancing factor (AEF) accelerates amyloid deposition. Researchers identified ubiquitin as the active component of AEF, demonstrating its role in inducing amyloid formation in mice during inflammatory stress.

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Area of Science:

  • Biochemistry
  • Immunology
  • Pathology

Background:

  • Amyloidosis is a group of diseases characterized by the deposition of amyloid proteins.
  • Amyloid enhancing factor (AEF) is known to accelerate amyloid deposition but its identity remains unclear.

Purpose of the Study:

  • To identify the molecular nature of AEF.
  • To investigate the role of ubiquitin in amyloid deposition.

Main Methods:

  • Purification of AEF from mouse liver and spleen extracts using gel filtration, SDS-PAGE, and ion exchange chromatography.
  • Mouse bioassays to assess the amyloid-inducing activity of purified peptides.
  • Immunochemical characterization using antibodies against ubiquitin.
  • Immunocytochemistry to detect ubiquitin levels in leucocytes.

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Main Results:

  • A 5.5 kDa peptide with AEF activity was isolated and identified as ubiquitin.
  • Anti-ubiquitin antibodies neutralized AEF activity.
  • Increased ubiquitin levels were observed in leucocytes during amyloid deposition.
  • Ubiquitin and its adducts demonstrated dose-dependent AEF activity.

Conclusions:

  • Ubiquitin is identified as the active component of AEF.
  • Ubiquitin plays a significant role in accelerating AA amyloid deposition.
  • Serum amyloid protein A and ubiquitin may converge to induce amyloidosis under inflammatory conditions.