[Production of antihaptene antisera. Comparison of methods of immunisation (author's transl)]

Five immunogenic substances were injected to rabbits. The method of immunisation by the intradermal route at 30 to 50 different points, with low doses of immunogen, without booster, does not seem to lead to production of antiserum of great specificity. The choice of the dose of immunogen to be injected is difficult, owing to our ignorance of the immunogenic dose for a given immunogen. The method of immunisation by the subcutaneous route with high doses followed by a booster, leads to more constant results, comparable to those obtained by the first method to which one may associate boosters by the subcutaneous route. The titers, affinities and specificities of the antisera thus produced are sufficient for the development of radio-immunoassay. The characteristics of the antisera obtained, related to the quality of the immunogen, do not seem to depend on the method of immunisation.