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A rapid assay for the measurement of Na+,K(+)-ATPase inhibitors
1Department of Chemical Pathology, Chinese University of Hong Kong, Shatin.
Clinical Biochemistry
|February 1, 1992
Abstract:
A rapid and automated assay for inhibitors of Na+, K(+)-ATPase was developed by determining the amount of inorganic phosphorus (Pi) released by Na+,K(+)-ATPase in a centrifugal analyzer. This method avoids long incubation, strong acids and centrifugation as in the conventional manual method. The coefficients of variation of intra- and inter-assay at ouabain concentration 0.5 mumol/L were 1.0 and 1.4%, respectively. The method is quick, reproducible and easy compared with current techniques.