Related Experiment Videos
[Construction and identification of eukaryotic expression vector for sense and antisense human telomerase reverse
Lai-bao Sun1, Cheng-rong Li, Jian-ming Wen
1Shenzhen Children's Hospital, Shenzhen 518026, China. sunlb361@163.com
Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA
|September 18, 2003
Summary
Researchers successfully created sense and antisense human telomerase reverse transcriptase (hTERT) eukaryotic expression vectors. These tools are vital for investigating telomerase regulation and developing gene therapies for cancers.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Context:
- Telomerase plays a crucial role in cellular immortalization and cancer.
- Understanding hTERT regulation is key for developing targeted cancer therapies.
- Eukaryotic expression vectors are essential tools for gene function studies.
Purpose:
- To construct eukaryotic expression vectors for both sense and antisense human telomerase reverse transcriptase (hTERT).
- To facilitate further research into the regulation of telomerase activity.
- To support the development of gene therapy strategies for malignant tumors.
Summary:
- A 210-bp fragment of hTERT cDNA was amplified using RT-PCR from HeLa cell total RNA.
- The amplified fragment was cloned into pGEM-T and subcloned into the pcDNA3.1 eukaryotic expression vector.
- Sense and antisense hTERT eukaryotic expression vectors were successfully constructed and confirmed by sequencing and restriction digestion.
Impact:
- Provides essential tools for studying hTERT gene function and regulation.
- Enables advancements in gene therapy approaches for cancer treatment.
- Contributes to the fundamental understanding of telomere biology and its implications in oncology.