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[Vitrification of mouse blastocysts using two kinds of straw systems]
Liang Zhu1, Chen Lou, Min-zhen Huang
1Center of Assisted Reproduction, Nanfang Hospital, Guangzhou 510515, China. 9_9gy@163.com
Objective:
To compare open pulled straws with conventional sorans system for the purpose of establishing an effective vitrification method for mouse blastocysts.
Methods:
With open pulled straws (OPS) and conventional straws, vitrifi- cation of mouse blastocysts was performed, and the survival and hatching rates of the blastocysts were observed after thawing.
Results:
After vitrification, the embryo survival in the OPS group (82.3%) was significantly higher than conventional straw group (68.6%, P<0.01), and the hatching rate was also higher (70.3%, P<5
Conclusions:
Vitrification of mouse blastocysts using OPS results in higher survival and hatching rates as compared with conventional straw method.