Related Experiment Video
Updated: Sep 18, 2026
Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
Published on: December 28, 2015
[The importance of flow cytometry and the cell proliferation index in choroidal melanoma]
M-L Ranty1, J-C Quintyn, P Courville
1Service d'Anatomie et de Cytologie Pathologiques, CHU Charles-Nicolle, Rouen. rantyml@hotmail.com
Purpose:
To evaluate the prognostic value of DNA ploidy and proliferative activity in metastasis occurrence after enucleation for choroidal melanomas.
Methods:
This retrospective study investigated 14 patients (eight males, six females) using flow cytometry and immunohistochemistry with the Ki67 antibody.
Results:
Two of the tumors were aneuploid, 12 were diploid. The proliferation index was between 0% and 16%. Two tumors progressed to metastatic disease; both were diploid and had a low proliferation index. They were mixed cell type. The median observation period was 3 years and 9 months.
Conclusion:
DNA content and proliferative activity are not correlated with metastasis occurrence. Sclera invasion is the best prognostic factor.
