Related Experiment Video

Updated: Sep 18, 2026

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
08:52

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

Cytomegalovirus infections in allogeneic stem cell recipients after reduced-intensity or myeloablative conditioning

J Schetelig1, O Oswald, N Steuer

  • 1Charité Campus Virchow Klinikum, Humboldt-Universität zu Berlin, Medizinische Klinik m.S. Hämatologie und Onkologie, Berlin, Germany.

Bone Marrow Transplantation
|September 18, 2003
PubMed

Insights

Reduced-intensity conditioning for hematopoietic stem cell transplantation (HSCT) did not significantly alter cytomegalovirus (CMV) infection rates compared to myeloablative conditioning. CMV seropositivity and graft-versus-host disease were key risk factors for CMV antigenemia.

Area of Science:

  • Hematology
  • Infectious Diseases
  • Transplantation Immunology

Background:

  • Cytomegalovirus (CMV) infections are a significant complication following hematopoietic stem cell transplantation (HSCT).
  • The intensity of pre-transplant conditioning regimens may influence the incidence of CMV infections post-HSCT.

Purpose of the Study:

  • To compare the incidence and kinetics of CMV infections after reduced-intensity conditioning (RIC) versus myeloablative conditioning (MAC) in HSCT recipients.
  • To identify risk factors for CMV antigenemia in HSCT patients.

Main Methods:

  • Prospective monitoring of 82 patients undergoing HSCT with either RIC (fludarabine, busulfan, ATG) or MAC.
  • Utilized CMV-PCR, CMV-antigenemia assays, and clinical observation for CMV detection.
  • Multivariate analysis to assess risk factors for CMV antigenemia.

Main Results:

  • Leukocyte and platelet engraftment were faster in the RIC group compared to the MAC group.
  • The incidence, onset, and peak values of CMV antigenemia and DNAemia did not differ significantly between RIC and MAC groups.
  • CMV seropositivity in recipients and acute graft-versus-host disease (GVHD) grade II-IV were significant risk factors for CMV antigenemia, but conditioning intensity was not.

Conclusions:

  • Reduced-intensity conditioning does not statistically alter the incidence or kinetics of CMV infections post-HSCT compared to myeloablative conditioning.
  • CMV seropositivity and acute GVHD are significant predictors of CMV antigenemia, highlighting the importance of recipient factors and immune status.

More Related Videos

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System10:24

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Published on: October 5, 2015

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus11:18

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus

Published on: May 7, 2012

Related Concept Videos

Cytomegalovirus Disease01:27

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...