Distribution and potential role of cytosolic water-soluble phosphodiesters in fish

The distribution of water-soluble phosphodiesters (WSPDEs) visible by nuclear magnetic resonance (NMR) in some intact tissues of rainbow trout (Oncorhynchus mykiss walbaum) and in perchloric extracts after partial purification was examined by (31)P NMR spectroscopy. The compounds of interest were serine ethanolamine phosphate (SEP), threonine ethanolamine phosphate (TEP), glycerophosphorylcholine (GPC), and glycerophosphorylethanolamine (GPE). TEP and SEP were mostly accumulated in the heart and less accumulated in the kidney of intact trout. After the extraction procedure, two additional minor resonances were visible and identified as GPC and GPE. The liver of trout contained large amounts of GPE. Similar investigations were conducted by (31)P NMR on hearts and kidneys of two elasmobranchs (Scyliorhinus canicula, Raja clavata) and four teleosts (Anguilla anguilla, Sparus auratus, Dicentrarchus labrax, Scophtlhalmus maximus); comparison with the trout data showed striking interspecies differences in the identity of WSPDEs. All teleosts, except eel and turbot, accumulated predominantly TEP. However, in elasmobranchs, first GPC and then GPE were the major compounds. Whatever the studied species, the relative abundances in the heart and kidney were similar. In the last two decades, two hypotheses were proposed to explain the occurrence of high levels of cytosoluble phosphodiesters: these compounds may constitute an index of phospholipid catabolism or a protective mechanism through which phospholipid levels are kept high. To test them and elucidate the role of these compounds in membrane phospholipid regulation in fish, we investigated the effects of two physiological stresses, that is, seawater adaptation and induced myocardial ischemia, on trout cytosolic phosphodiester levels. A 32.5-min ischemic stress caused no effect on SEP and TEP levels. On the contrary, significant osmotic stress induced changes in the PDEs levels: 2 d after transfer from freshwater to seawater or from seawater to freshwater, both tissues displayed a transient decrease of TEP; however, a 2-d stay in seawater after transfer from freshwater caused a rise in SEP concentration, whereas a 2-d stay in freshwater after transfer decreased SEP level. In conclusion, our experiments suggest a relationship between the high levels of cytosoluble phosphodiesters observed in some fish tissues and resistance to stress.