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Diagnosis of equid herpesviruses -1 and -4 by polymerase chain reaction
P C Sharma1, A A Cullinane, D E Onions
1Department of Veterinary Pathology, University of Glasgow Veterinary School, UK.
Equine Veterinary Journal
|January 1, 1992
Abstract:
The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses-1 and -4 (EHV-1 and EHV-4) DNA within nasopharyngeal swab samples from horses. Ninety-eight samples from suspected field cases and in-contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our results indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV-1 and EHV-4 infection.