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Recognition of SV40-VP2 in the infected cell by antipeptide antibodies

H J Streckert1, H J Sommerfeld, K Morgenroth

  • 1Department of Medical Microbiology and Virology, Ruhr-University Bochum, Federal Republic of Germany.

Archives of Virology
|January 1, 1992
PubMed

Antipeptide antibodies were elicited against two synthetic peptides corresponding to amino acids 47-55 and 98-103 of the structural protein VP 2 of SV 40. The induced antibodies proved to be VP 2-specific in an immunoblot. In immunofluorescence these antibodies showed a discrete nuclear and perinuclear staining pattern. In immune electron microscopy studies the induced antibodies did not bind to major virions suggesting that VP 2 is not present at the surface of SV 40 particles.

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