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Updated: Aug 16, 2026

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
Published on: November 28, 2011
Specific insulin-like growth factor-I receptors on circulating bovine mononuclear cells
X Zhao1, B W McBride, L M Trouten-Radford
1Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.
Abstract:
We have examined the presence and properties of specific receptors for IGF-I on bovine mononuclear cells. Competitive binding studies showed that binding of [125I]IGF-I to mononuclear cells was inhibited by unlabelled peptides with the rank of IGF-I greater than IGF-II greater than insulin. The binding of [125I]IGF-I was a function of the cell concentration. Equilibrium dissociation constant and receptor concentration values for the average of 9 adult cows were 1.13 +/- 0.11 nM and 108.9 +/- 24.1 fMol/10(7) cells, respectively. Moreover, IGF-I stimulated thymidine incorporation into bovine mononuclear cells in the absence of serum and phytohemagglutinin (PHA). The existence of specific and functional IGF-I receptors on circulating bovine mononuclear cells would provide an easily accessible source for studying IGF-I receptor changes in the bovine, both in physiologic and pathologic states.
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