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Testosterone destroys the transcortin-receptor complex
S A Krupenko1, O A Strel'chyonok
1Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, Belarus.
Biochemical and Biophysical Research Communications
|April 15, 1992
Abstract:
Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10(-5) M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. The specificity of this effect is confirmed by its abrogation in the presence of cortisol. The testosterone effect has been used for the elution of transcortin receptor from affinity column. The receptor retained transcortin-binding capacity after the elution and removal of testosterone. Characteristics of the receptor obtained by testosterone elution were identical with those of the transcortin eluted preparation.