Related Experiment Videos
GLOBIC: a very fast microcomputer program for fingerprinting, characterization and comparison of long nucleotide
Computer Applications in the Biosciences : CABIOS
|February 1, 1992
Summary
The GLOBIC program rapidly compares DNA sequences by analyzing oligonucleotide compositions, not raw sequences. It generates visual maps to reveal sequence relationships, aiding genomic analysis.
Area of Science:
- Bioinformatics
- Computational Biology
- Genomics
Background:
- Comparing and characterizing large DNA sequences is crucial for genomic research.
- Existing methods can be time-consuming for analyzing extensive nucleotide sequences.
Purpose of the Study:
- To introduce GLOBIC, a novel microcomputer-based program for rapid sequence comparison and characterization.
- To develop a method for fingerprinting DNA sequences efficiently.
Main Methods:
- GLOBIC analyzes local nucleotide or short oligonucleotide compositions instead of entire sequences.
- It utilizes an IBM-AT microcomputer for high-speed processing.
- Two-dimensional contour line maps visualize sequence similarity and relationships.
Main Results:
- GLOBIC can compare, characterize, and fingerprint sequences as small as 0.1 megabase in minutes.
- The program generates visual maps illustrating similarities between sequences, self-comparisons, complementary sequences, and random sequences.
- A defined vocabulary translates map patterns into sequence relationship meanings.
Conclusions:
- GLOBIC offers a fast and effective approach to DNA sequence comparison and characterization.
- The program's visual mapping and interpretation facilitate understanding of genomic sequence relationships.
- Demonstrated applications include genomic sequences from bacteriophage T7, adenovirus type-2, and Epstein-Barr virus.