Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Curcumin inhibits nitrite-induced methemoglobin formation.

M K Unnikrishnan1, M N Rao

  • 1Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Manipal, India.

FEBS Letters
|April 20, 1992
PubMed
Summary

Curcumin prevents hemoglobin oxidation to methemoglobin by nitrite. This protective effect is lost if curcumin is added after the reaction

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational use and regulatory stewardship for paediatric cough syrups in India: A public health imperative.

Indian journal of medical ethics·2026
Same author

Rising antimicrobial resistance: Impending post-antibiotic era?

Medical journal, Armed Forces India·2025
Same author

Hutchinson-Gilford progeria syndrome: unraveling the genetic basis, symptoms, and advancements in therapeutic approaches.

Therapeutic advances in rare disease·2024
Same author

India's latest essential medicines list: gaps, strengths and opportunities in evidence-based decision-making.

BMJ evidence-based medicine·2023
Same author

Effectiveness of blended learning in pharmacy education: An experimental study using clinical research modules.

PloS one·2021
Same author

Phonon-based partition of (ZnSe-like) semiconductor mixed crystals on approach to their pressure-induced structural transition.

Scientific reports·2020

Area of Science:

  • Biochemistry
  • Oxidative Stress
  • Hemoglobinopathies

Background:

  • Nitrite induces hemoglobin oxidation to methemoglobin.
  • This process involves an autocatalytic stage.
  • Superoxide radicals may play a role in this oxidation.

Purpose of the Study:

  • To investigate the protective effect of curcumin against nitrite-induced methemoglobin formation.
  • To determine the optimal timing for curcumin intervention.
  • To explore the mechanism underlying curcumin's protective action.

Main Methods:

  • In vitro study of hemoglobin oxidation kinetics.
  • Addition of curcumin at different time points during the reaction.
  • Assessment of methemoglobin formation spectrophotometrically.

Main Results:

  • Curcumin significantly inhibited hemoglobin oxidation to methemoglobin when added before or during the early stages.
  • Protection was not observed when curcumin was added after the autocatalytic phase.
  • Curcumin's known superoxide scavenging ability is proposed as the mechanism.

Conclusions:

  • Curcumin demonstrates a time-dependent protective effect against nitrite-induced methemoglobin formation.
  • The findings suggest curcumin's efficacy is linked to its ability to neutralize superoxide radicals during the critical autocatalytic stage.
  • This highlights curcumin's potential as a therapeutic agent in conditions involving oxidative damage to hemoglobin.

Related Experiment Videos