Jove
Visualize
Contact Us

Related Experiment Videos

Divalent cations and extracellular matrix receptor function during newt epidermal cell migration.

J T Mahan1, D J Donaldson

  • 1Department of Anatomy and Neurobiology, University of Tennessee, Memphis 38163.

Journal of Cell Science
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced Surface Partitioning of Nitrate Anion in Aqueous Bromide Solutions.

The journal of physical chemistry letters·2015
Same author

Reply to "Comment on 'Photolysis of Polycyclic Aromatic Hydrocarbons on Water and Ice Surfaces' and on 'Nonchromophoric Organic Matter Suppresses Polycyclic Aromatic Hydrocarbon Photolysis in Ice and at Ice Surfaces'".

The journal of physical chemistry. A·2015
Same author

Heterogeneous photochemistry in the atmosphere.

Chemical reviews·2015
Same author

Red-light initiated atmospheric reactions of vibrationally excited molecules.

Physical chemistry chemical physics : PCCP·2013
Same author

Heterogeneous photooxidation of fluorotelomer alcohols: a new source of aerosol-phase perfluorinated carboxylic acids.

Environmental science & technology·2013
Same author

Can we model snow photochemistry? Problems with the current approaches.

The journal of physical chemistry. A·2013
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Calcium (Ca) is essential for keratinocyte cell-cell connections during migration, while calcium, magnesium (Mg), or manganese (Mn) support cell-substratum interactions for migration on fibronectin and fibrinogen.

Area of Science:

  • Cell Biology
  • Dermatology
  • Biochemistry

Background:

  • Keratinocyte migration is crucial for skin wound healing and tissue regeneration.
  • The roles of divalent cations (calcium, magnesium, manganese) in cell migration and adhesion are complex and substrate-dependent.

Purpose of the Study:

  • To investigate the distinct roles of calcium (Ca), magnesium (Mg), and manganese (Mn) in keratinocyte migration.
  • To determine how these cations influence cell-cell adhesion and cell-substratum interactions on different extracellular matrix proteins.

Main Methods:

  • Skin explants cultured on fibronectin (FN), fibrinogen (FGN), or collagen-coated dishes.
  • Culture in serum-free media with varying divalent cations: Ca/Mg, Ca-only, Mg-only, or Mn-only.
  • Analysis of keratinocyte migration patterns and halo formation around explants.

Related Experiment Videos

Main Results:

  • Calcium (Ca) was required for contiguous cell sheets (halos) on all substrates, indicating its role in cell-cell adhesion.
  • Magnesium (Mg)-only conditions promoted significant cell migration but resulted in dissociated cells, suggesting Mg facilitates cell-substratum interaction.
  • Manganese (Mn)-only supported limited migration, and migration on collagen was notably reduced in Ca-only conditions compared to FN and FGN.

Conclusions:

  • Exogenous Ca is essential for maintaining keratinocyte cell-cell connections during migration.
  • Cell-substratum interactions driving migration can utilize Ca, Mg, or Mn, with substrate-specific differences observed.
  • Migration on collagen exhibits distinct cation requirements compared to fibronectin and fibrinogen.