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Infection of Chang cells with hepatitis C virus using hepatic biopsy specimens from patients with chronic hepatitis
T Ozeki1, K Hikiji, K Funakoshi
13rd Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
International Journal of Experimental Pathology
|February 1, 1992
Abstract:
The presence of hepatitis C virus sequence was detected in liver tissue extracts by the polymerase chain reaction (PCR) method using primers of non-coding region in six out of eight cases with chronic hepatitis seropositive for Chiron's antibody. Subsequently, liver extracts from these cases were added to cell cultures of Chang cells for 3 days. The liver extracts of the six cases positive for PCR appeared to infect the Chang cells.