[Quantitative direct gas-liquid chromatography for rapid diagnosis of anaerobic infections]

Quantitative direct gas-liquid chromatography (GLC) was performed on 74 specimens of surgical infections and relevant findings were compared with cultural results. In the present study, significant amounts of multiple volatile fatty acids (VFA) and/or succinate were found as markers of anaerobic infections. At least one of the VFA (propionic, isobutyric, butyric, isovaleric or valeric acid) greater than or equal to 0.1 mumol/ml and/or succinate greater than or equal to 0.3 mumol/ml was strong evidence for anaerobic infections. More than 3.5 mumol/ml of butyric acid was interpreted as indication of the presence of Fusobacterium spp. Succinate greater than or equal to 0.3 mumol/ml was associated with the presence of Bacteroides fragilis.