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Related Experiment Videos

A cellular function is required for pseudorabies virus envelope glycoprotein processing and virus egress.

M E Whealy1, A K Robbins, F Tufaro

  • 1Du Pont Merck Pharmaceutical Company, Viral Diseases Research, Wilmington, Delaware 19880-0328.

Journal of Virology
|June 1, 1992
PubMed
Summary

The gro29 mouse cell mutant impairs herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV) glycoprotein export and virus egress. This suggests a critical host protein is essential for efficient viral propagation.

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Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • The mouse L-cell mutant gro29 exhibits defects in herpes simplex virus type 1 (HSV-1) virion egress and glycoprotein export.
  • Alphaherpesviruses, including HSV-1 and pseudorabies virus (PRV), rely on host cell machinery for replication and egress.

Purpose of the Study:

  • To investigate the role of the gro29 mutation in pseudorabies virus (PRV) infection.
  • To determine if the cellular defect in gro29 cells affects other alphaherpesviruses.
  • To elucidate the specific steps in viral glycoprotein export and egress impacted by the gro29 mutation.

Main Methods:

  • Infection of gro29 cells with PRV.
  • Analysis of viral glycoprotein synthesis and export kinetics.
  • Assessment of infectious particle formation and release.

Related Experiment Videos

  • Use of a secreted viral membrane protein variant to study Golgi export.

    • Main Results:

    • PRV-infected gro29 cells displayed defects in glycoprotein export, infectious particle formation, plaque formation, and virus egress.
    • Export of viral glycoproteins from the endoplasmic reticulum to the Golgi was impaired, and Golgi export was blocked late in infection.
    • Export of a secreted viral protein from the Golgi out of the cell was also defective.
    • PRV formed plaques poorly on gro29 cells, with limited early virus release followed by blocked egress.

    Conclusions:

    • The gro29 mutation affects multiple steps in viral glycoprotein export and virus egress, suggesting involvement of a single protein utilized at various stages or multiple proteins.
    • The identified host cell protein is crucial for the efficient propagation of both HSV-1 and PRV.
    • The gro29 cell line serves as a valuable tool for studying host-pathogen interactions in alphaherpesvirus replication.