Thyroid hormone affects the hydrolysis of inositol phospholipids in the rat hypothalamus

¹First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.

|January 6, 1992

Abstract:

We have attempted to elucidate the effect of thyroid hormone on phospholipase C-linked inositol phospholipid hydrolysis in the rat hypothalamus. Hypothalamic slices of each animal, euthyroid control, hypothyroid, and thyroxine (T4)-supplemented hypothyroid rats were labeled with [3H]myoinositol in the presence of 5 mM LiCl, and then incubated for 60 min in KHG buffer containing either vehicle or 1 mM ouabain, a Na-K ATPase inhibitor. Hypothyroidism caused a significant increase in both basal and ouabain-stimulated accumulation of [3H]inositol phosphate ([3H]IP) in hypothalamic slices, whereas supplement with T4 to hypothyroid rats resulted in a complete restoration of hypothalamic [3H]IP formation to the value of euthyroid control. The present results indicate that thyroid hormone affects phospholipase C-linked inositol phospholipid hydrolysis in the hypothalamus, suggesting that negative feedback action of thyroid hormone may occur at a post-receptor site in the hypothalamus.

Keywords:

inositol phosphate phospholipase C thyroxine supplementation neuroendocrine regulation

Related Experiment Videos

Thyroid hormone affects the hydrolysis of inositol phospholipids in the rat hypothalamus

Related Concept Videos

The Neuropeptide Cyclo(His-Pro) Levels are Increased in Diabetic Ketoacidosis and Fall with Treatment in Parallel with Levels of Glucagon.

A possible mechanism of the nucleus accumbens and ventral pallidum 5-HT1B receptors underlying the antidepressant action of ketamine: a PET study with macaques.

Effects of valsartan and amlodipine on home blood pressure and cardiovascular events in Japanese hypertensive patients: a subanalysis of the VART.

Mental fatigue-induced decrease in levels of several plasma amino acids.

Relationship between plasma cyclo (His-Pro), a neuropeptide common to processed protein-rich food, and C-peptide/insulin molar ratio in obese women.

Expression of thyrotropin receptors in rat thymus.

Left DLPFC activation in the association between psychological resilience and depressive symptoms in college students.

Prior trauma shapes the effects of psilocybin on stress sensitization in mice.

Single-housed, but not pair-housed, male and female rats demonstrate social conditioned place preference.

Maternal separation reshapes layer-specific PV-PNN architecture in the mouse barrel cortex.

Nrf2 overexpression ameliorates cognitive deficits and neuronal damage in APP/PS1 mice via attenuation of Aβ deposition and neuronal ferroptosis.

Peripheral endotoxemia is associated with microglial activation, neuronal apoptosis, and amyloid precursor protein accumulation.

Cytologic and Cytochemical Characterization of Blue-Green Neutrophilic Inclusions in a Dog with Suspected Transfusion-Associated Hemolysis.

Intravenous Lipid Emulsion Attenuates Hepatic, Renal, Cerebral and Testicular Histopathological Injury but Not Renal Dysfunction or Cardiac Histological Change in an Acute Haloperidol Overdose Model in Rats: Implications for Veterinary Toxicology.

Functional and Transcriptional Responses of Neutrophils from Dogs Living Under Different Housing Conditions Following In Vitro Lipopolysaccharide Stimulation.

Single-Cell RNA Sequencing of Uterine Tissue from a 70-Day-Old Female Rabbit: Cellular Composition and Fibroblast-Associated Networks.

A Streptococcus iniae-associated outbreak in hybrid sturgeon (Acipenser baerii ♀ × Acipenser schrenckii ♂): pathological features and transcriptomic responses to experimental challenge.

Multi-GPU parallel framework for DOI-enabled long axial field-of-view PET image reconstruction.

Related Experiment Videos

Thyroid hormone affects the hydrolysis of inositol phospholipids in the rat hypothalamus

Abstract:

Related Concept Videos

Related Articles