Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Targeted gene disruption in Epstein-Barr virus.

M A Lee1, O J Kim, J L Yates

  • 1Department of Human Genetics, Roswell Park Cancer Institute (a unit of New York State Department of Health), Buffalo 14263.

Virology
|July 1, 1992
PubMed
Summary

We developed a novel method for inserting genetic markers into the Epstein-Barr virus (EBV) genome using homologous recombination. This technique enhances EBV genetic engineering efficiency and facilitates the creation of targeted EBV mutants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pan-Asian adapted ESMO Clinical Practice Guidelines for the diagnosis, treatment and follow-up of patients with pancreatic cancer.

ESMO open·2025
Same author

Pan-Asian adapted ESMO Clinical Practice Guidelines for the diagnosis, treatment and follow-up of patients with metastatic colorectal cancer.

ESMO open·2023
Same author

Pan-Asian adapted ESMO Clinical Practice Guidelines for the diagnosis treatment and follow-up of patients with localised colon cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Anterior resection syndrome: a randomized clinical trial of a 5-HT3 receptor antagonist (ramosetron) in male patients with rectal cancer.

The British journal of surgery·2021
Same author

Capecitabine plus oxaliplatin versus gemcitabine plus oxaliplatin as first-line therapy for advanced biliary tract cancers: a multicenter, open-label, randomized, phase III, noninferiority trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2019
Same author

The severity of periodontitis and metabolic syndrome in Korean population: The Dong-gu study.

Journal of periodontal research·2017

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Epstein-Barr virus (EBV) is a human herpesvirus associated with various cancers.
  • Efficient methods for targeted genetic manipulation of the EBV genome are crucial for research.

Purpose of the Study:

  • To develop a versatile method for inserting selective markers into any EBV genome region.
  • To enhance the efficiency of generating recombinant EBV strains.

Main Methods:

  • Homologous recombination using plasmids to insert selective markers into the EBV B95-8 strain genome.
  • Isolation of EBV recombinants as G418-resistant B-cell clones or subclones.
  • Utilizing the EBV productive replication origin (oriLyt) on plasmids to stimulate recombination.

Related Experiment Videos

Main Results:

  • The method allows targeted insertion of markers into any EBV genome region.
  • Inclusion of oriLyt on plasmids increased recombinant virus yield approximately 100-fold.
  • Long flanking DNA segments and larger plasmid size favored double-sided recombination.

Conclusions:

  • A robust method for EBV genetic modification via homologous recombination was established.
  • The use of oriLyt significantly boosts the efficiency of generating recombinant EBV.
  • This technique facilitates the creation of EBV mutants, such as those with disrupted BALF2 open-reading frame.