Related Experiment Videos
Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system
T P McGonigal1, M C Turon, K Komar-Hartnett
1Department of Corporate Molecular Biology, Abbott Laboratories, North Chicago, IL.
Virus Research
|April 1, 1992
Abstract:
The gene coding for the major outer capsid protein (VP7) of simian rotavirus SA-11 has been expressed in a baculovirus-insect cell system. The resulting protein is 35 kDa and is primarily associated with the endoplasmic reticulum. Neutralizing SA-11 polyclonal antiserum and VP7 monospecific antiserum reacted specifically with the expressed gene product. Antiserum derived against the recombinant VP7 protein neutralized SA-11 rotavirus infectivity in a fluorescent focus assay.