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Multiple sequence elements are involved in RNA 3' end formation in spleen necrosis virus
1McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Gene Expression
|January 1, 1992
Summary
The polyadenylation signal
Area of Science:
- Molecular Biology
- Gene Regulation
- Virology
Background:
- Polyadenylation signals (PAS) are crucial for mRNA processing.
- The distance between the transcription start site (cap site) and PAS influences PAS function in some viruses.
Purpose of the Study:
- To investigate the distance-dependent function of polyadenylation signals.
- To compare the polyadenylation signals of spleen necrosis virus (SNV) and simian virus 40 (SV40).
Main Methods:
- Construction and analysis of chimeric polyadenylation signals.
- Deletion analysis of the SNV poly(A) sequence.
- Comparison of poly(A) signal function across different viral and cellular genes.
Main Results:
- SNV polyadenylation signal function is distance-dependent, unlike the SV40 late polyadenylation signal.
- Deletions in the SNV poly(A) sequence did not affect its distance-dependent function.
- Chimeric SNV/SV40 polyadenylation signals exhibited intermediate behavior, as did signals from mouse alpha-globin and herpes simplex virus thymidine kinase genes.
Conclusions:
- Multiple sequence elements contribute to the distinct functions of SNV and SV40 polyadenylation signals.
- The observed intermediate behavior suggests conserved mechanisms or shared elements in polyadenylation signal function across different gene systems.