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Related Experiment Videos

An immunoradiometric assay for squirrel monkey prolactin.

J G Scammell1, A L Haynes, L E Williams

  • 1Department of Pharmacology University of South Alabama, College of Medicine, Mobile 36688.

Laboratory Animal Science
|June 1, 1992
PubMed
Summary

A new assay reliably measures squirrel monkey prolactin using human prolactin antibodies. Ketamine HCl significantly increased prolactin levels, unlike pentobarbital sodium anesthesia.

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Area of Science:

  • Endocrinology
  • Primate Physiology

Background:

  • Measuring squirrel monkey prolactin is challenging due to a lack of specific antibodies.
  • Existing assays for human prolactin may not cross-react with primate prolactins.

Purpose of the Study:

  • To adapt a commercial human prolactin immunoradiometric assay for squirrel monkey prolactin measurement.
  • To investigate the effect of ketamine HCl on prolactin secretion in squirrel monkeys.

Main Methods:

  • Utilized a commercially available immunoradiometric assay with two monoclonal antibodies (MAb I and II) against human prolactin.
  • Performed Western blot analysis on squirrel monkey pituitary homogenates using [125I]-MAb II.
  • Administered ketamine HCl and pentobarbital sodium to squirrel monkeys to assess effects on serum prolactin.

Main Results:

  • Prolactin immunoreactivity curves in squirrel monkey samples paralleled human standards, indicating epitope conservation.
  • Western blot detected both glycosylated (26 kD) and nonglycosylated (23 kD) forms of squirrel monkey prolactin.
  • Ketamine HCl (30 mg/kg) caused a >4-fold increase in serum prolactin, while pentobarbital sodium had no effect.

Conclusions:

  • The adapted assay is a reliable and sensitive method for quantifying immunoreactive squirrel monkey prolactin.
  • The assay demonstrates specificity for primate prolactins, not recognizing sheep or rat prolactin.
  • Ketamine HCl is a potent stimulator of prolactin release in squirrel monkeys.

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