Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An immunoradiometric assay for squirrel monkey prolactin.

J G Scammell1, A L Haynes, L E Williams

  • 1Department of Pharmacology University of South Alabama, College of Medicine, Mobile 36688.

Laboratory Animal Science
|June 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility of a Composite Measure of Pulmonary Vascular Impedance and Application to Patients with Chronic RV Failure Post LVAD Implant.

Cardiovascular engineering and technology·2023
Same author

Incidental Findings from 16,400 Brain MRI Examinations of Research Volunteers.

AJNR. American journal of neuroradiology·2023
Same author

Functional hydraulic sectoring in grapevines as evidenced by sap flow, dye infusion, leaf removal and micro-computed tomography.

AoB PLANTS·2021
Same author

Diurnal variations of androgens in sexually mature male Bolivian squirrel monkeys (Saimiri sciureus) during the breeding season.

American journal of primatology·2020
Same author

Detection and cultivation of intestinal trichomonads of squirrel monkeys (Saimiri sciureus).

American journal of primatology·2020
Same author

Determination of lactogenic activity in the serum of the squirrel monkey (Saimiri boliviensis) using the Nb2 lymphoma bioassay.

American journal of primatology·2020

A new assay reliably measures squirrel monkey prolactin using human prolactin antibodies. Ketamine HCl significantly increased prolactin levels, unlike pentobarbital sodium anesthesia.

Area of Science:

  • Endocrinology
  • Primate Physiology

Background:

  • Measuring squirrel monkey prolactin is challenging due to a lack of specific antibodies.
  • Existing assays for human prolactin may not cross-react with primate prolactins.

Purpose of the Study:

  • To adapt a commercial human prolactin immunoradiometric assay for squirrel monkey prolactin measurement.
  • To investigate the effect of ketamine HCl on prolactin secretion in squirrel monkeys.

Main Methods:

  • Utilized a commercially available immunoradiometric assay with two monoclonal antibodies (MAb I and II) against human prolactin.
  • Performed Western blot analysis on squirrel monkey pituitary homogenates using [125I]-MAb II.
  • Administered ketamine HCl and pentobarbital sodium to squirrel monkeys to assess effects on serum prolactin.

Related Experiment Videos

Main Results:

  • Prolactin immunoreactivity curves in squirrel monkey samples paralleled human standards, indicating epitope conservation.
  • Western blot detected both glycosylated (26 kD) and nonglycosylated (23 kD) forms of squirrel monkey prolactin.
  • Ketamine HCl (30 mg/kg) caused a >4-fold increase in serum prolactin, while pentobarbital sodium had no effect.

Conclusions:

  • The adapted assay is a reliable and sensitive method for quantifying immunoreactive squirrel monkey prolactin.
  • The assay demonstrates specificity for primate prolactins, not recognizing sheep or rat prolactin.
  • Ketamine HCl is a potent stimulator of prolactin release in squirrel monkeys.