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A maize cryptic Ac-homologous sequence derived from an Activator transposable element does not transpose.

J Y Leu1, Y H Sun, Y K Lai

  • 1Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.

Molecular & General Genetics : MGG
|June 1, 1992
PubMed
Summary

A novel maize DNA sequence, cAc-11, shows homology to Activator (Ac) transposable elements but lacks transposase activity. This cryptic element is hypermethylated and non-transposing, suggesting it is a remnant Ac or Dissociation (Ds) element.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Transposable elements (TEs) like Activator (Ac) are abundant in the maize genome.
  • Understanding the nature and behavior of Ac-like sequences is crucial for maize genetics.

Purpose of the Study:

  • To characterize a newly isolated cryptic Ac-like DNA sequence from maize, designated cAc-11.
  • To determine the molecular features and transposition potential of cAc-11.

Main Methods:

  • DNA isolation and sequencing of the cAc-11 element from maize inbred line 4Co63.
  • Bioinformatic analysis to compare cAc-11 with known Ac and Dissociation (Ds) elements.
  • Analysis of cAc-11 methylation status and transposition in the presence of active Ac.

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Main Results:

  • cAc-11 exhibits over 90% homology to Ac sequences, with characteristic inverted terminal repeats and target site duplications.
  • cAc-11 lacks a significant open reading frame for transposase and contains a tandem repeat, suggesting aberrant transposition.
  • Maize genomic DNA homologous to cAc-11 is widespread, but cAc-11 itself is hypermethylated and non-transposing.

Conclusions:

  • cAc-11 represents a non-functional remnant of an Ac or Ds transposable element in maize.
  • Its hypermethylated state and lack of transposition indicate epigenetic silencing.
  • The prevalence of cAc-11 homologs suggests a significant role of remnant TEs in the maize genome.