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Incorporation of BrdU in a DNA fragment may affect protein-DNA interactions in a site-dependent manner
G Kumar1, A K Sharma, K Jayaraman
1Department of Molecular Biology and Genetics, Wayne State University, School of Medicine, Detroit, Michigan 48201.
Oncogene
|July 1, 1992
Abstract:
DNA in which BrdU has been randomly incorporated is used to identify specific DNA-binding proteins. We show here that the location of BrdU incorporation in a DNA fragment can significantly affect specific protein-DNA interactions and thereby identification of DNA-binding proteins. We suggest that a radioactive labeled unmodified DNA, rather than BrdU-incorporated DNA, be used for identifying DNA-binding proteins by UV cross-linking.