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[Redundant nucleotide-sequences in DNA from wheat (author's transl)]
Zeitschrift Fur Naturforschung. Section C, Biosciences
|January 1, 1976
Summary
DNA in dormant and germinated embryos shows distinct reassociation kinetics. Changes in DNA renaturation, specifically Cot1/2 values for the redundant fraction, are detectable within 24 hours of germination.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Context:
- Embryonic development involves significant molecular changes.
- DNA reassociation kinetics is a method to study genome structure and complexity.
- Understanding changes in DNA during germination is crucial for developmental biology.
Purpose:
- To investigate differences in DNA reassociation kinetics between dormant and germinated embryos.
- To quantify changes in the redundant DNA fraction during early germination.
Summary:
- DNA from dormant and germinated embryos exhibits differential thermal denaturation and reassociation kinetics.
- A reduction in renaturation, indicated by Cot1/2 of the redundant fraction, is observed within 24 hours of embryo germination.
Impact:
- This study reveals dynamic alterations in DNA structure during embryonic germination.
- Findings contribute to understanding gene regulation and genome organization during plant development.