Related Experiment Videos
Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey
1Dept. of Genetics, Martin Luther University, Halle/S., Germany.
Plant Molecular Biology
|June 1, 1992
Abstract:
The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.