Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultrastructural changes in in vitro ageing spinach chloroplasts.

G Kulandaivelu, D O Hall

    Zeitschrift Fur Naturforschung. Section C, Biosciences
    |January 1, 1976
    PubMed
    Summary

    In vitro ageing spinach chloroplasts swell due to thylakoid membrane changes. Bovine serum albumin stabilizes membranes, while freezing preserves thylakoid structures despite chloroplast breakage.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The stability of electron transport in in vitro chloroplast membranes.

    Photosynthesis research·2014
    Same author

    Photochemical activities and organization of photosynthetic apparatus of C3 and C 4 plants grown under different light intensities.

    Photosynthesis research·2014
    Same author

    Photochemical activities and organization of photosynthetic apparatus of C3 and C 4 plants grown under different light intensities.

    Photosynthesis research·2014
    Same author

    Species specific chlorophyll a fluorescence-temperature profile at high temperatures in the leaves.

    Photosynthesis research·2014
    Same author

    Immobilization of Anabaena azollae from Azolla filiculoides in polyvinyl foam for ammonia production in a photobioreactor system.

    World journal of microbiology & biotechnology·2014
    Same author

    The potential applications of cyanobacterial photosynthesis for clean technologies.

    Photosynthesis research·2013

    Area of Science:

    • Plant biology
    • Cell biology
    • Biochemistry

    Background:

    • Chloroplasts are vital organelles for photosynthesis in plants.
    • Understanding chloroplast stability during storage is crucial for research and applications.
    • In vitro ageing affects cellular structures, including chloroplast membranes.

    Purpose of the Study:

    • To investigate the ultrastructural changes in spinach chloroplasts during in vitro ageing.
    • To identify factors influencing chloroplast membrane stability.
    • To evaluate preservation methods for thylakoid structures.

    Main Methods:

    • Spinach chloroplasts were subjected to in vitro ageing under controlled conditions.
    • Ultrastructural analysis was performed using electron microscopy.
    • The effects of bovine serum albumin and low-temperature storage (77 K) were examined.

    Main Results:

    • Prolonged storage led to chloroplast swelling and increased thylakoid membrane thickness and spacing.
    • Lipid release was observed to accompany membrane thickening.
    • Bovine serum albumin addition stabilized chloroplast membrane structures.
    • Storage at 77 K preserved thylakoid structures effectively, despite complete chloroplast breakage.

    Conclusions:

    • In vitro ageing induces significant ultrastructural alterations in spinach chloroplasts.
    • Membrane lipid composition and external stabilizing agents influence chloroplast stability.
    • Low-temperature storage offers a method for preserving thylakoid integrity.

    Related Experiment Videos